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            Goat IgG Fc Antibody Peroxidase Conjugated
Rabbit Polyclonal
            
                605-4303
        
        
            
                2 mg
        
            
                
                    Lyophilized
            
            
                
                ELISA
            
                    
                
                Goat
            
                            
                
                 Rabbit
            
                
            
        
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Product Details
                    
                    
Rabbit Anti-Goat IgG Fc Antibody Peroxidase Conjugated - 605-4303                    
                
                
                    
                    
Goat IgG F(c) Antibody, Rb-a-Goat IgG F(c) Peroxidase conjugated, goat IgG F(c) Peroxidase Antibody in rabbit, Rabbit  anti-goat IgG F(c) peroxidase conjugated Secondary Antibody                    
                
                
                    
                    
Rabbit                    
                
                
                    
                    
IgG Fc                    
                
                
                    
                    
Peroxidase (HRP)                    
                
                
                    
                    
Polyclonal                    
                
                
                    
                    
IgG                    
                
            Target Details
                    
                    
Goat                                            
                
                
                    
                    
Native Protein                                            
                
                
                    
                    
Anti-Goat IgG F(c) was produced by repeated immunization with Goat IgG F(c) fragment in rabbit.                                            
                
                
                    
                    
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab) .                                            
                
                Application Details
                        
                        
ELISA
                        
                    
                    
                        
                        
Goat IgG F(c) peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 is suggested for this product.
                        
                    
                                                    Formulation
                        
                        
2.0 mg/mL by UV absorbance at 280 nm                        
                    
                    
                        
                        
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2                        
                    
                    
                        
                        
0.01% (w/v) Gentamicin Sulfate.  Do NOT add Sodium Azide!                        
                    
                    
                        
                        
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free                        
                    
                    
                        
                        
1.0 mL                        
                    
                    
                        
                        
Restore with deionized water (or equivalent)                        
                    
            Shipping & Handling
                    
                    
Ambient                    
                
                
                    
                    
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.                    
                
                
                    
                    
Expiration date is one (1) year from date of receipt.                    
                
        
                Anti-Goat IgG F(ab')2 Peroxidase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH.  F(ab')2 molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 molecules.
            
        
            This product is for research use only and is not intended for therapeutic or diagnostic applications.  Please contact a technical service representative for more information.  All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin.  Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation.  All properties listed are typical characteristics and are not specifications.  All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control.  All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale.  Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc.  If you require a commercial license to use this material and do not have one, then return this material, unopened to:  Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
        
     
                         
     
     
     
    