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Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
Donkey Polyclonal
2 References
611-705-127
1 mg
Liquid (sterile filtered)
WB, ELISA, Dot Blot
Rabbit
Donkey
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Product Details
Donkey Anti-Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-705-127
Donkey anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Donkey anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation
Donkey
IgG (H&L)
Alkaline Phosphatase (AP)
Polyclonal
IgG
Target Details
Rabbit
Native Protein
Anti-Rabbit IgG (H&L) was produced by repeated immunization with Rabbit IgG whole molecule in donkey
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Application Details
Dot Blot, ELISA
WB
- View References
Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
Formulation
1 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Shipping & Handling
Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of receipt.
Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody generated in donkey detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Vallejo J, Hardin CD. (2004). Metabolic organization in vascular smooth muscle: distribution and localization of caveolin-1 and phosphofructokinase. Am J Physiol Cell Physiol.
Applications
WB, IB, PCA
Williams D. (2003). Production and Biocompatibility of Spider Silk Proteins in Goat Milk. Med Device Technol.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
