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Streptavidin Antibody Fluorescein Conjugated
Rabbit Polyclonal
2 References
200-4295
200-402-095S
200-402-095
20 mg
25 µL
100 µg
Lyophilized
Liquid (sterile filtered)
Lyophilized
Cellular Assay
Streptavidin
Rabbit
Shipping info:
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Product Details
Anti-Streptavidin (RABBIT) Antibody Fluorescein Conjugated (BULK ORDER) - 200-4295
rabbit anti-Streptavidin Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin FITC Antibody
Rabbit
Fluorescein (FITC)
Polyclonal
IgG
Target Details
Streptavidin
Native Protein
Streptavidin (Streptomyces avidinii)
Anti-Streptavidin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
Application Details
Cellular Assay
- View References
Anti-Streptavidin Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Streptavidin antibody is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7). With a dissociation constant (Kd) on the order of ≈10−14 mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH. Anti-STREPTAVIDIN antibody is ideal for investigators involved in GFP and Epitope research.
Tsai, RK et al. (2010). Self inhibition of phagocytosis: the affinity of 'marker of self' CD47 for SIRPalpha dictates potency of inhibition but only at low expression levels. Blood Cells, Molecules & Diseases
Applications
Phagocytosis Assay
Tsai, RK. et al. (2008). Inhibition of "self" engulfment through deactivation of myosin-II at the phagocytic synapse between human cells. The Journal of Cell Biology
Applications
Phagocytosis Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.