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TGF beta 1 Antibody
Rabbit Polyclonal
1 References
600-401-432
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC
Human
Rabbit
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Product Details
Anti-TGF beta 1 (RABBIT) Antibody - 600-401-432
rabbit anti-TGFB1 antibody, rabbit anti-TGF beta 1 antibody, rabbit anti-TGF ß 1 antibody, Transforming growth factor beta-1, Latency-associated peptide, LAP, TGFB 1
Rabbit
Polyclonal
IgG
Target Details
TGFB1 - View All TGFB1 Products
Human
Conjugated Peptide
TGF beta 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length).
Rabbit Anti-TGF beta 1 affinity purified antibody is directed against human TGFb1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from most mammalian sources based on 100% homology for the immunogen sequence. Cross-reactivity is expected with TGFb1 from non-mammalian sources as only a single amino acid residue change is found within the immunogen sequence from many other organisms.
Application Details
ELISA, WB
IHC
- View References
Anti-TGF beta 1 Antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 12.5 kDa in size corresponding to reduced mature TGFb1 by western blotting in the appropriate cell lysate or extract.
Formulation
1.19 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, including three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule; the amino acid sequence between human and mouse differs by only one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues, such as placenta and kidneys.
Filgueiras LR et al. (2014). Sepsis-induced lung inflammation is modulated by insulin. BMC Pulm Med.
Applications
IHC, ICC, Histology
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
