ModDetect™ Phosphorothioate Clone PS03
Mouse Monoclonal PS03 IgG2a kappa
200-301-MU9
100 µg
Liquid (sterile filtered)
ELISA
Mouse
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Product Details
ModDetect™ Phosphorothioate Clone PS03 - 200-301-MU9
Phosphorothioate, PS, ASO, anti-sense oligonucleotide, siRNA, RNA therapeutic
Mouse
Monoclonal
IgG2a
Target Details
Other
ModDetect™ Phosphorothioate Clone PS03 was prepared from cell culture supernatant produced by repeated immunizations with a proprietary oligo sequence.
This Protein A purified ModDetect™ Phosphorothioate Clone PS03 is directed against phosphorothioate modified backbone. This product was Protein A purified from monospecific supernatant.
Application Details
ELISA
ModDetect™ Phosphorothioate Clone PS03 has been validated for use in ELISA. In antigen-down or immunometric ELISAs, the ModDetect™ Phosphorothioate Clone PS03 preferentially detects phosphorothioate modified phosphate groups over native phosphodiester sugar-phosphate backbones. It is also likely to be functional in IF and IHC assays, however, its sensitivity, specificity, and conditions for optimal use in these assays has not yet been determined. All assays should be optimized by the user.
Formulation
1.040 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Shipping & Handling
Dry Ice
Store vial at -20° C or below prior to opening. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Current studies focusing on RNA therapeutics use as potential drugs antisense oligonucleotides (ASO), short interfering RNA (siRNA), and micro RNA (miRNA). The choice of oligonucleotide chosen for a specific treatment is dependent on many factors. However, all oligo therapeutics must be modified in some manner, as unmodified nucleotides are difficult to identify and are highly susceptible to degradation by endogenous nucleases. Modifications can be created within the sugar-phosphate backbone (first generation), the ribose sugar (second and third generation), or at multiple locations (third generation). Antibodies to modified oligonucleotides are useful for basic immunoassays including, ELISA, IHC, and IF. In addition, several types of toxicology assays (immunogenicity assays), such as antibody-drug assays (ADA) or other pharmacokinetic (PK) and pharmacodynamic (PD) studies require oligonucleotide antibodies as analytical tools for general research and preclinical trial experiments. Antibodies to an oligonucleotide therapeutic allow researchers to assess cellular uptake, tissue distribution, and can serve as a positive control for immunogenicity studies.
THE MATERIALS PROVIDED WITH THIS CERTIFICATE OF ANALYSIS (MATERIALS) ARE FOR RESEARCH USE ONLY AND SUBJECT TO A MATERIAL EVALUATION AGREEMENT.
BY USING THIS PRODUCT, THE USER AGREES TO BE BOUND BY THE TERMS OF THE MATERIAL EVALUATION AGREEMENT. If the user is not willing to accept the terms of the agreement and the product is unused, Rockland will accept return of the unused product and provide the user with a full refund. Pursuant to the agreement, the user may use the product for research use only.
NO TRANSFER OR COMMERCIAL USE OF THIS PRODUCT IS ALLOWED. Commercial use means a use of the product in exchange for consideration, including use in the manufacture of a product, in a clinical trial or other testing regulated by a government agency (e.g. FDA, EMEA, EPA, etc.) including ADA and clearance assays, use of the product in the provision of services, and the resale of the product. Rockland reserves all other rights not permitted under the license granted herein, including the rights which are embodied in other products or materials derived from the use of the Materials or know-how embodied in the Materials.
NO RIGHT IS GRANTED UNDER THE LICENSE TO MODIFY OR OTHERWISE CREATE DERIVATIVES OR VARIATIONS OF THE PRODUCT OR TO USE ROCKLAND’S KNOW-HOW OR OTHER INTELLECTUAL PROPERTY FOR OTHER USES. No other use of this product is authorized without the express written prior consent of Rockland, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, therapeutic uses, or any type of consumption by or application to humans or animals.
For uses outside the agreement including any diagnostic, therapeutic or commercial use, please contact Rockland for supply and licensing information at licensing@rockland.com.