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            Alpha-Tubulin Antibody
Rabbit Polyclonal
            
                600-401-Y07
        
        
            
                100 µg
        
            
                
                    Liquid (sterile filtered)
            
            
                
                WB, ELISA, IHC, IF
            
                    
                
                Human, Mouse, Rat
            
                            
                
                 Rabbit
            
                
    
        
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Product Details
                    
                    
Anti-Alpha-Tubulin (RABBIT) Antibody - 600-401-Y07                    
                
                
                    
                    
Alpha-tubulin Antibody, TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2                    
                
                
                    
                    
Rabbit                    
                
                
                    
                    
Polyclonal                    
                
                
                    
                    
IgG                    
                
            Target Details
                    
                    
TUBA3C                         - View All TUBA3C Products
                            
                    
                
                
                    
                    
Human, Mouse, Rat                                            
                
                
                    
                    
Conjugated Peptide                                            
                
                
                    
                    
Anti-Alpha-tubulin antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid peptide near the N-terminus of human alpha-tubulin                                            
                
                
                    
                    
Anti-Alpha-tubulin antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Alpha-tubulin antibody is human, mouse and rat reactive.                                            
                
                
                Application Details
                        
                        
ELISA, IF, IHC, WB
                        
                    
                    
                        
                        
Anti-Alpha-tubulin Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 50 kDa in Western Blots of specific cell lysates and tissues.
                        
                    
                                                    Formulation
                        
                        
1.0 mg/mL by UV absorbance at 280 nm                        
                    
                    
                        
                        
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2                        
                    
                    
                        
                        
0.02% (w/v) Sodium Azide                        
                    
                    
                        
                        
None                        
                    
            Shipping & Handling
                    
                    
Dry Ice                    
                
                
                    
                    
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.                    
                
                
                    
                    
Expiration date is one (1) year from date of receipt.                    
                
        
                Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families.  Along with beta-tubulins, alpha-tubulins are the major components of microtubules.  These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape.  Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter.  Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.
            
        
            This product is for research use only and is not intended for therapeutic or diagnostic applications.  Please contact a technical service representative for more information.  All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin.  Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation.  All properties listed are typical characteristics and are not specifications.  All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control.  All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale.  Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc.  If you require a commercial license to use this material and do not have one, then return this material, unopened to:  Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
        
     
                         
                                             
                                             
                                            